Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858.
Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145.
|Petite Kit Includes||Quantity|
|SignalStain® DAB Diluent||1 x 12 ml|
|SignalStain® DAB Chromogen Concentrate||1 x 360 µl|
|Small Kit Includes||Quantity|
|SignalStain® DAB Diluent||1 x 120 ml|
|SignalStain® DAB Chromogen Concentrate||1 x 3.6 ml|
To prepare the DAB working solution, add 1 drop (30 μl) SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent and mix well before use. Working solutions are stable for up to 14 days when stored at 4ºC or up to 5 days when stored at room temperature (approximately 25ºC). While the solution may change color over that time period, it will have no effect on the quality of the staining.
*Please note that when using 8059P, a dropper for the chromogen concentrate is not included. Use a pipet to add 30 μl of SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent.
Important: DAB is a suspected carcinogen. Good laboratory practices should be followed, and appropriate protective equipment should be used when handling DAB, including gloves, protective eyeware, and a lab coat. Dispose in accordance with local regulations.
After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 1-10 minutes generally provides an acceptable staining intensity.
Wash slides in water for 5 minutes.
Counterstain, if desired, and mount coverslips with either a permanent mounting media or an aqueous-based mounting media. Please note, permanent mounting requires dehydration through ethanol and clearing through xylene.
Each SignalStain® DAB Substrate Kit includes enough DAB Diluent and DAB Chromogen Concentrate to stain either 1200 (#8059S) or 120 (#8059P) slides.
Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 12 months upon receipt if the reagents are not combined.
The SignalStain® DAB Substrate Kit contains all of the necessary reagents to prepare a working solution of diaminobenzidine (DAB) for staining tissue sections. The DAB working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a brown reaction product.
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