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9223
ATF-2 Control Cell Extracts
Experimental Controls

ATF-2 Control Cell Extracts #9223

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  1. WB

Western blot analysis of ATF-2 Control Cell Extracts using Phospho-ATF-2 (Thr69/71) Antibody #9225 (upper) and ATF-2 (20F1) Rabbit mAb #9226 (lower).

Product Usage Information

Boil for 3 minutes prior to use. Load 20 µl of phosphorylated and nonphosphorylated ATF-2 Control Cell Extracts per lane.

Storage:

Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

Product Description

Nonphosphorylated ATF-2 Control Cell Extracts: Total extracts from NIH/3T3 cells, to serve as a negative control. Supplied in SDS Sample Buffer.

Phosphorylated ATF-2 Control Cell Extracts: Total extracts from NIH/3T3 cells, treated with Anisomycin #2222 at 25 ug/ml for 30 minutes to serve as a positive control. Supplied in SDS Sample Buffer.

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

  1. Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
  2. Gupta, S. et al. (1995) Science 267, 389-93.
  3. van Dam, H. et al. (1995) EMBO J 14, 1798-811.
  4. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

To Purchase # 9223S
Product # Size Price
9223S
200 µl  (10 western blots) N/A

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