|Petite Kit Includes||Quantity|
|SignalFire™ Elite ECL Reagent A||1 x 10 ml|
|SignalFire™ Elite ECL Reagent B||1 x 10 ml|
|Small Kit Includes||Quantity|
|SignalFire™ Elite ECL Reagent A||1 x 50 ml|
|SignalFire™ Elite ECL Reagent B||1 x 50 ml|
(a) Wash membrane-bound HRP (antibody conjugate) at least three times for 5 minutes in TBS/T. It is very important to thoroughly wash the membrane prior to substrate incubation.
(b) Prepare 1X SignalFire™ Elite ECL Reagent by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
(c) Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
*Avoid repeated exposure to skin (see enclosed Material Safety Data Sheet or refer to our website for further information).
Each SignalFire™ Elite ECL Reagent (A and B) is a 2X concentrate; there is no need to further dilute in water when the two reagents are combined.
Store at 4°C.
|Activity||Storage: Store at 4ºC. This product is stable for 12 months. DO NOT store Reagents A and B pre-mixed. Reagents A and B should be combined just prior to exposing membranes.|
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