Cat. # | Size | Qty. | Price |
---|---|---|---|
2915T | 1 Kit (9 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
Wnt3a (C64F2) Rabbit mAb 2721 | 20 µl |
|
M | 42 | Rabbit IgG |
Wnt5a/b (C27E8) Rabbit mAb 2530 | 20 µl |
|
H | 45 | Rabbit IgG |
Phospho-LRP6 (Ser1490) Antibody 2568 | 20 µl |
|
H | 210 | Rabbit |
Dvl2 (30D2) Rabbit mAb 3224 | 20 µl |
|
H M R Mk | 90 to 95 | Rabbit |
LRP6 (C47E12) Rabbit mAb 3395 | 20 µl |
|
H M | 180, 210 | Rabbit IgG |
Dvl3 Antibody 3218 | 20 µl |
|
H M R Hm Mk Mi B | 88 to 93 | Rabbit |
Naked1 Antibody 2262 | 20 µl |
|
H | 59, 61 | Rabbit |
Naked2 (C67C4) Rabbit mAb 2073 | 20 µl |
|
H | 59, 61 | Rabbit |
Axin1 (C76H11) Rabbit mAb 2087 | 20 µl |
|
H M | 110 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Information
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a, Glu176 of human Wnt5a, Met988 of human LRP6, Gly176 of human Dvl2, and Ala217 of human Naked2. The monoclonal antibody for Axin1 is produced by immunizing animals with recombinant human Axin1. The phospho-specific polyclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1490 of human LRP6. The total polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human Dvl3 and Gln227 of human Naked1. Polyclonal antibodies are purified by Protein A and peptide affinity chromotagraphy.
The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (2). Wnt members bind to the Frizzled family of proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway requires a coreceptor from proteins belonging to the low-density lipoprotein receptor (LDLR) family, such as LRP5 and LRP6 (4). Upon stimulation with Wnt, LRP6 is phosphorylated by kinases such as GSK-3 and CK1 and subsequently recruits Axin to the membrane (5-7). In the absence of Wnt stimulation, the multidomain scaffold proteins Axin1 and Axin2 negatively regulate Wnt signaling. Axin1 complexes with APC, GSK-3β, Dvl and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (8-9). The Disheveled (Dsh) proteins (Dvl1, Dvl2 and Dvl3) inhibit glycogen synthase kinase-3β, thereby promoting β-catenin stabilization (4,10). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dsh proteins and directing Dsh activity towards the planar cell polarity pathway (11-12).
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