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Cleaved caspase-3 product listings, product citations, comparison tables and educational resources for apoptosis signaling from Cell Signaling Technology.
Caspase Cleavage Substrate Proteomics
Find out the best control cell extracts to use as positive or negative controls in your western blots, when studying apoptosis, autophagy, and mitophagy.
Expert-reviewed interactive pathway providing a current overview of Death Receptor Signaling.
Study the intricate machinery of the apoptosis signaling pathway. Click here to learn more and gain insights into programmed cell death mechanisms.
Products and Related Resources for Cell Death and Viability SARS-CoV-2 Research
Necrosis has been classically defined as an unprogrammed form of cell death that occurs in response to overwhelming chemical or physical insult.
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
Overview of apoptosis signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources for apoptosis research.
The Caspase-4 Antibody #4450 recognizes an epitope that is present in both the full-length protein and the p20 subunit. Therefore, this antibody is predicted to detect both targets. However, we have not confirmed the reactivity of the Caspase-4 Antibody #4450 with the p20 subunit at endogenous levels. The exact protein load of recombinant, active caspase-4 shown in the western image on the product datasheet for the Caspase-4 Antibody #4450 has not been recorded. However, we typically load 1-5 ng of recombinant protein for such experiments.
Regulation of cell death due to viral infection is important for host survival. In this series, we look at pathways regulated through cellular responses to viruses.
We have validated the Caspase-3 Activity Assay Kit #5723 in human and mouse cell lines. However, as long as there is active caspase-3 present, this kit is predicted to work with most cell lines. Depending on the cell type and the incubation time applied in the assay, 0.5 - 2x10^5 cells/well (or 100 ug/well of total lysate protein) is sufficient for most experimental setups. For best results, cell number or lysate concentration titrations are recommended. Because caspase-7 shares the same substrate sequence as caspase-3, this kit also detects caspase-7 activity.
Compare Cell Signaling Technology's Cleaved Caspase antibodies against one another to determine optimal applications.
Additional internal testing has shown that the Caspase-3 (8G10) Rabbit mAb #9665 recognizes both Caspase-3 and Caspase-7. When this antibody was released to the market, we were unaware of the homology between the two proteins. We sincerely apologize for any inconvenience this may have caused.
A scientific resource for the DED protein domain containing information on structure, function, and domain binding during apoptosis.
A scientific resource for the BIR protein domain containing information on structure, function, and domain binding in apoptosis.
The Inflammasome pathway is essential for the innate immune response, but it can also contribute to the onset of a number of diseases. Learn more here.
Apoptosis is a regulated form of programmed cell death (PCD) that occurs without external influence.
For imaging purposes, we recommend using Cleaved Caspase-3 (Asp175) Antibody #9661 or Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579 on human cells and frozen tissues. We have noted cytoplasmic background with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 in some human samples.
Pyroptosis is a type of programmed necrotic cell death activated by infections from bacteria, viruses, fungi or protozoa in the presence of PAMPs or DAMPs
For imaging purposes, we recommend using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 when working with rodent tissue. We have noted non-specific labeling in specific sub-types of healthy cells in fixed-frozen mouse tissues (e.g. pancreatic alpha-cells) and nuclear background in rat samples with Cleaved Caspase-3 (Asp175) Antibody #9661 and Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579.
Caspase-3 Activity Protocol: easy to follow directions describing the step by step experimental procedure.
Cell viability is a measure of the proportion of live, healthy cells within a population. Assays to assess cell viability measure metabolic activity, ATP content, cell proliferation, or membrane integrity.
We have performed side-by-side testing of the Caspase-3 Activity Assay Kit #5723, with and without PMSF, and find that the assay performs better without the addition of PMSF. The PathScan Sandwich ELISA Lysis Buffer (1X) #7018 has been optimized for use with the Caspase-3 Activity Assay Kit #5723 without the addition of supplemental inhibitors, such as PMSF.
These markers can be used to study necroptosis and pyroptosis, forms of regulated necrotic cell death that share morphological features with necrosis.
Products and Related Resources for Virus Detection and Innate Immune Activation SARS-CoV-2 Research
Activated by pro-inflammatory signaling, ischemic injury or viral infection, necroptosis is a programmed regulated form of necrosis requiring RIP3 and MLKL
Expert-reviewed interactive pathway providing a current overview of Inhibition of Apoptosis Signaling.
Explore the mitochondrial apoptosis pathway and its role in programmed cell death. Read more about this essential pathway here.