Need a conjugated antibody that you don’t see in the Cell Signaling Technology® (CST®) catalog? Save time and avoid pitfalls, like the low yields associated with do-it-yourself (DIY) kits. We can conjugate it for you.
CUSTOM ANTIBODY CONJUGATION FORM
| Conjugation Type: Examples | Service Tier | Service Includes |
|---|---|---|
| Fluorophores Type I: Alexa Fluor®, Cyanine (CyDye®) and Pacific Blue™ dyes | Basic | Conjugation + removal of free dye |
| Tier I | Degree of labeling (DOL) + Basic Service | |
| Tier II | Validation1 + Tier I Service | |
| Tier III | Stability testing + Tier II Service | |
| Fluorophores Type II: Phycoerythrin (PE), PE-CyDye® tandems (e.g. PE-Cy®7), and allophycocyanin (APC) | Basic | Conjugation + purification |
| Tier I | Validation1 + Basic Service | |
| Tier II | Stability testing + Tier I Service | |
| Haptens: Biotin, Digoxigenin (DIG), DNP, and Dansyl | Basic | Conjugation + removal of free biotin |
| Tier I | Validation2 + Basic Service | |
| Beads: Sepharose® and Magnetic | Basic | Conjugation + removal of free antibody |
| Tier I | Validation3 + Basic Service | |
| Enzymes: Horseradish peroxidase (HRP) | Basic | Conjugation + Purification |
| Tier I | Validation2 + Basic Service | |
| Oligonucleotide 4 | Basic | Conjugation + Purification |
| Tier I | Biophysical Characterization + Basic Service |
DIY conjugation kits look good on paper, but are you getting your money’s worth? Inefficiencies caused by suboptimal labeling, interference with specificity, and/or destabilization/degradation can negatively affect performance. Another drawback is the loss of antibody during conjugation steps, which can mean finishing with significantly less functional antibody than you started with.
With Custom Conjugation Services from Cell Signaling Technology (CST), you can avoid these time-consuming pitfalls and get the full amount of antibody you pay for.
Comparison of PE conjugation of Helios (D8W4X) XP® Rabbit mAb using CST custom conjugation method (blue) or conjugated using a competitor’s DIY PE conjugation kit (green). Flow cytometeric analysis of RL (dashed lines), a low-expressing cell line, and Jurkat (solid lines), a high-expressing cell line. The calculated signal-to-noise ratio is shown (above).
Comparison of HRP conjugation of Phospho-Akt (Ser473) (193H12) Rabbit mAb conjugated using CST custom conjugation method (blue) or conjugated using a competitor’s DIY HRP conjugation kit (green), performed by ELISA assay. The relationship between the protein concentration of lysate (x-axis) from MCF7 cells untreated (dashed lines) or treated with human IGF I (solid lines) and the absorbance at 450 nm is shown (y-axis). The signal-to-noise ratio was calculated at the 0.625 mg/ml lysate concentration (in associated table).
Comparison of HRP conjugation of GAPDH (D16H11) XP® Rabbit mAb using CST conjugation method or conjugated using a another company’s DIY HRP conjugation kit. Western blot analysis of extracts from C6 and THP-1 cells were performed with concentration-matched antibody conjugates. These data demonstrate the increased signal specificity observed when using CST custom conjugation versus a DIY HRP conjugation kit.
Regarding Alexa Fluor conjugation, this product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected]. Alexa Fluor® is a registered trademark of Life Technologies Corporation. Cy, Cy7, CyDye. Pacific Blue is a registered trademark of Molecular Probes, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
