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Custom Antibody Conjugation

Custom Antibody Conjugation

Need a conjugated antibody that you don’t see in the Cell Signaling Technology (CST) catalog? We can conjugate it for you.

  • Antibodies are validated, optimized, and tested for stability with the same standards of the conjugated antibodies available in the catalog
  • Save time and avoid pitfalls like the low yields associated with DIY kits – you only pay for the final, conjugated antibody
  • Fast turnaround times –basic conjugations typically ship within 10 days
  • When you need to validate the conjugated antibody yourself, we also offer faster, lower-cost basic conjugation services

CUSTOM ANTIBODY CONJUGATION INQUIRIES FORM

Conjugation Services available:

Conjugation Type: Examples Service Tier Service Includes
Fluorophores Type I: Alexa Fluor®, Cyanine (CyDye®) and Pacific Blue dyes Basic Conjugation + removal of free dye
Tier I Degree of labeling (DOL) + Basic Service
Tier II Validation1 + Tier I Service
Tier III Stability testing + Tier II Service
Fluorophores Type II: Phycoerythrin (PE), PE-CyDye® tandems (e.g. PE-Cy®7), and allophycocyanin (APC) Basic Conjugation + purification
Tier I Validation1 + Basic Service
Tier II Stability testing + Tier I Service
Haptens: Biotin, Digoxigenin (DIG), DNP, and Dansyl Basic Conjugation + removal of free biotin
Tier I Validation2 + Basic Service
Beads: Sepharose® and Magnetic Basic Conjugation + removal of free antibody
Tier I Validation3 + Basic Service
Enzymes: Horseradish peroxidase (HRP) Basic Conjugation + Purification
Tier I Validation2 + Basic Service
  • 1 Standard validation application method is Flow Cytometry. Alternatives may increase timeline and pricing.
  • 2 Standard validation application method is Western Blot. Alternatives may increase timeline and pricing.
  • 3 Standard validation application method is IP. Alternatives may increase timeline and pricing.

Advantages of CST Custom Conjugation:

  • Flexibility: Tiered options range from basic antibody conjugation to full validation and stability testing of conjugates
  • Validation: Conjugates are tested in key applications using biologically relevant cell systems and controls, and verified for physical integrity using size exclusion chromatography
  • Optimization: Service options include choice of conjugation chemistries, removal of free dye and/or antibody purification, and identification of optimal degree of labeling (DOL) to ensure the best signal-to-noise ratio
  • Support: Application consultation and extensive technical support from the same scientists who produce and validate our commercially available antibody conjugates

In regards to Alexa Fluor conjugation, this product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected]. Alexa Fluor® is a registered trademark of Life Technologies Corporation. Cy, Cy7, CyDye, and Sepharose are registered trademarks of GE Healthcare. Pacific Blue is a registered trademark of Molecular Probes, Inc. Dylight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

DIY conjugation kits look good on paper, but are you getting your money’s worth? Inefficiencies caused by suboptimal labeling, interference with specificity, and/or destabilization/degradation can negatively affect performance. Another drawback: loss of antibody during conjugation steps can mean finishing with significantly less functional antibody than you started with.

With Custom Conjugation Services from Cell Signaling Technology (CST), you can avoid these time-consuming pitfalls and get the full amount of antibody you pay for.

Comparison of PE-conjugated antibody in flow cytometry

18-WFO-15801 Comparison of PE Conjugated Antibody in Flow Fever Chart

Comparison of PE conjugation of Helios (D8W4X) XP® Rabbit mAb using CST custom conjugation method (blue) or conjugated using a competitor’s DIY PE conjugation kit (green). Flow cytometeric analysis of RL-7 (dashed lines), a low-expressing cell line, and Jurkat (solid lines), a high-expressing cell line. The calculated signal-to-noise ratio is shown (below).

Comparison of HRP-conjugated antibody in ELISA

18-WFO-15801 DIY ELISA comp data-v.3

Comparison of HRP conjugation of Phospho-Akt (Ser473) (193H12) Rabbit mAb conjugated using CST custom conjugation method (blue) or conjugated using a competitor’s DIY HRP conjugation kit (green), performed by ELISA assay. The relationship between the protein concentration of lysate from MCF7 cells untreated (dashed lines) or treated with human IGF I (solid lines) and the absorbance at 450 nm is shown (left). The signal-to-noise ratio was calculated at the 0.625 mg/ml lysate concentration (right).

Comparison of HRP-conjugated antibody in western blot

18-WFO-15801-Comparison of HRP-conjugated antibody in Western Blot-v.2

Comparison of HRP conjugation of GAPDH (D16H11) XP® Rabbit mAb using CST conjugation method or conjugated using a competitor’s DIY HRP conjugation kit. Western blot analysis of extracts from C6 and THP-1 cells were performed with concentration-matched antibody conjugates. These data demonstrate that signal specificity is better maintained with CST custom conjugation.

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