Cat. # | Size | Qty. | Price |
---|---|---|---|
20218S | 100 µl |
|
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 113 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GluD2/GRID2 protein.
Glutamate receptor ionotropic, delta-1 and delta-2 (GluD1 and GluD2, respectively) are part of the delta family of ionotropic glutamate receptors (iGluRs). Ionotropic receptors, which are typically cation-permeable ligand-gated ion channels, mediate most excitatory transmission in the brain (1). iGluR classes are defined based on sequence identity and pharmacological properties and include α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, kainate receptors, and N-methyl-D-aspartate (NMDA) receptors (2). Although GluDs were identified based on sequence identity and their structure is related to other iGluRs, GluDs do not bind glutamate and are unlikely to form ion channels like other iGluRs (3).
GluD1 and GluD2 share 56% sequence homology. While GluD2 expression is highest in the cerebellum and likely regulates motor function, GluD1 is expressed throughout the forebrain. While GluDs may not function like typical ligand-gated ion channels, they bind to other synaptic proteins and may function to alter subcellular expression of synaptic proteins, including other iGluRs as well as synaptic adhesion proteins. Genetic studies in mice suggest GluDs function in higher-order functions, including learning and memory, as well as addiction.
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