Cat. # | Size | Qty. | Price |
---|---|---|---|
5419S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 135 |
Source/Isotype | Mouse IgG2b |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 262
Human
Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human ZFX.
The ZFX (X-linked zinc finger protein) gene is expressed from the inactive X chromosome and is structurally similar to its homologue on the Y chromosome, ZFY (1). Transcripts of ZFX and ZFY encode proteins composed of a highly acidic amino-terminal domain and a carboxy-terminal zinc-finger motif that is commonly associated with nucleic acid-binding proteins (2). Both ZFY and ZFX are probable transcriptional activators and may function in sex determination (3). Multiple alternatively spliced transcript variants of ZFX, encoding different isoforms, have been identified and may be functionally distinct (2). Conditional gene targeting studies in mouse have suggested ZFX is also required for self-renewal of embryonic and hematopoietic stem cells (4). ZFX has also been suggested to play a role in proliferation and expansion of B cells, and could contribute to lymphocyte homeostasis (5).
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