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Western Blotting Application Solutions Kit
WB & IP Reagents
Buffer Kit

Western Blotting Application Solutions Kit #12957

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From sample preparation to detection, everything you need for a more efficient and economical western blot is now found in one easy kit. We get our WB results using these reagents, now you can too.

To Purchase # 12957S
Product # Size Price
1 Kit  (10 western blots) N/A

Product Includes Quantity
Cell Lysis Buffer (10X) 9803 15 ml
30X Reducing Agent (1.25 M DTT) 1 ml
3X Blue Loading Buffer 8 ml
Prestained Protein Marker, Broad Range (11-190 kDa) 13953 100 µl
BSA 9998 12 g
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
PMSF 8553 34.84 mg
Tris-Glycine SDS Running Buffer (10X) 4050 300 ml
Nitrocellulose Sandwiches 12369 10 Pack
Tris-Glycine Transfer Buffer (10X) 12539 300 ml
Tris Buffered Saline with Tween® 20 (TBST-10X) 9997 300 ml
Nonfat Dry Milk 9999 50 g
SignalFire(TM) ECL Reagent A 50 ml
SignalFire(TM) ECL Reagent B 50 ml

Reagents Not Included

  1. Primary Antibody
  2. 1X PBS (#9808, 20X)
  3. Reverse Osmosis Deionized (RODI) Water
  4. Isopropyl alcohol
  5. BCA Protein Assay (#7780) (optional)
  6. Biotinylated Protein Ladder Detection Pack (#7727) (optional)
  7. Methanol
  8. 1X TBS (#12498, 10X) (optional)
  9. Streptavidin-HRP (#3999) (if necessary)


All components in this kit are stable for at least 12 months past the reference date indicated on the component label when stored at the recommended temperature and left unused. Upon receipt, BSA and 3X Blue Loading Buffer should be removed from #13005S and stored at 4ºC and at room temperature, respectively. The 30X Reducing Agent and remaining #13005S components should be stored at -20ºC. All #13131S reagents should be stored at room temperature. IMPORTANT: Please refer to the primary antibody datasheet or product webpage for recommended primary antibody dilution buffer and recommended antibody dilution.

Product Description

The Western Blotting Application Solutions Kit is designed to conveniently provide reagents needed for western blotting, from cell lysis to protein detection. The reagents in this kit are thoroughly validated with our primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents to run 10 mini-gels and complete western blot assays with either rabbit or mouse primary antibodies. All reagents in this kit are available individually.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalFire is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

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